Hypocholesterol Effect of lndegenous Probiotic Culture and Yoghurt in Sprague Dawley Rats

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Published: Jun 4, 2023
Keywords:
probiotic, lactic acid bacteria, yoghurt, hypocholesterolemic effect

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Lily Arsanti Lestari
Staf pengajar Program Studi Gizi Kesehatan, Fakultas Kedokteran UGM
Eni Harmayani
Staf pengajar Jurusan Teknologi Pangan dan Hasil Pertanian, FTP UGM
Y. Marsono
Staf pengajar Jurusan Teknologi Pangan dan Hasil Pertanian, FTP UGM

Abstract

Probiotic was believed as an agent of lowering serum cholesterol level. However, the information about this is still controversy. Lactobacillus sp Dad13 is an indigenous probiotic culture isolated from traditional fermented milk. In vitro study proved that Lactobacillus sp Dad13 posseses the ability to assimilate cholesterol and deconjugate bile acids, however in vivo study hasn’t been done yet. The purpose of this research was to study the ability of Lactobacillus sp Dad13 and yoghurt which was supplemented with Lactobacillus sp Dad13 to reduce serum cholesterol level. Twenty four male Sprague Dawley rats, 2 months old were divided into four groups and fed with an AIN-93 diet ad libitum. Rats were fed with standard diet (1 wk) and hypercholesterol diet (1 wk). At the end of the week, lipid profile was measured. Each group were then treated with 10% sterile skimmed milk, as control, Lactobacillus sp Dad13 culture, yoghurt, and yoghurt which supplemented with Lactobacillus sp Dad13 culture 1 ml each. The treatments were given by force feeding. The treatments were assigned for 4 wk and at the end of treatments, serum lipid profile, physical, chemical, and microbiological properties of cecal contents i.e. weight, water contents, pH, cholesterol, and total lactic acid bacteria were analyzed. The results showed that total cholesterol, high density lipoprotein (HDL), triglyceride (TG), low density lipoprotein (LDL), and LDL/HDL ratio decreased significantly (P<0,05). The highest reduction of total cholesterol, approximately 39,8%, was found in the group treated with suspension of Lactobacillus sp Dad13 culture. Probiotics culture feeding increased lactic acid bacteria population in cecal contents. Cholesterol level of cecal contents corresponds with low cholesterol level of serum. It can be concluded that Lactobacillus sp Dad13, an indigenous probiotic culture, proved to have as hypocholesterolemic effect and can be developed as functional food.

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Hypocholesterol Effect of lndegenous Probiotic Culture and Yoghurt in Sprague Dawley Rats. (2023). TEMU ILMIAH NASIONAL PERSAGI, 1, 334-341. https://www.tin.persagi.org/index.php/tin/article/view/143
Issue
Vol. 1 (2005): PROSIDING Temu Ilmiah, Kongres PERSAGI XIII dan Festival Gizi Tahun 2005
Section
3. SIMPOSIA TIN PERSAGI 2005 - Pangan Fungsional
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How to Cite

Hypocholesterol Effect of lndegenous Probiotic Culture and Yoghurt in Sprague Dawley Rats. (2023). TEMU ILMIAH NASIONAL PERSAGI, 1, 334-341. https://www.tin.persagi.org/index.php/tin/article/view/143

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